General anaesthetic photolabels are used to find the molecular binding sites of anaesthetics, such as GABA receptors, protein kinase and tubulin. Involvement of anaesthetics with voltage-dependent channels for negative ions and the mitochondria has been demonstrated by photolabelling. At clinical concentrations anaesthetics can effect the functioning of proteins. Photolabels can attach to residues or individual molecules lining protein pockets, and thus indicate the presence of a dynamic ligand in a pocket.
Posts Tagged ‘tubulin’
Inhalational anaesthetic-binding proteins in neuronal membrane Jin Xi, Maryellen Eckenhoff, Roderic Eckenhoff et al, University of Pennsylvania & Amersham Biosciences :: The Journal of Biological Chemistry, Vol. 279, No. 19, May 7 2004, pp. 19628-19633 :: http://www.jbc.org/content/279/19/19628.full.pdf+html Summary and review of the above paper In this study 34 binding sites were identified for the anaesthetic halothane. Within this group, mitochondrial proteins especially those Read more […]
Atomic water channel controlling remarkable properties of a single brain microtubule: Correlating single protein to its supramolecular assembly Satyajit Sahu & Anirban Bandyopadhyay et al, National Institute for Materials Science, Japan Biosensors and Bioelectronics 47 141-148, 21 February 2013 Summary and review of the above paper INTRODUCTION: The authors say the prior to this paper the properties of tubulin and microtubules were not extensively studies using the up-to-date technologies Read more […]